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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Microbiol Methods. 2013 Aug 19;95(2):149–155. doi: 10.1016/j.mimet.2013.08.007

Table 3.

Total number of sequence reads and percent aligning to chloroplasts with the various forward primers.

50:50 Mock mixture A. constructor L. dispar
Primer combination # reads %chloroplast # reads %chloroplast # reads %chloroplast
27F/519R 661 55.4 1839 67 374 39.4
799F/1392R 7966 1.66 10759 0.37 2208 0.36
799F-tags/1392R 9764 2.47 3176 1.95 1675 6.87
799F-mod2/1392R 4390 1.09 8586 0.02 2798 0.11
799F-mod3/1392R 8657 0.88 8772 0 2307 0
799F-mod6/1392R 6717 0.76 8548 0 2115 0
799F-mod7/1392R 6370 0.63 6638 0 1795 0
799F-thio/1392R 10133 2.34 N/A N/A 254 0
799F-mod7thio/1392R 5196 2.1 N/A N/A 865 0