Table 1. Nasal shedding (as log10 PCR EID50 equivalents/mL) of an avian influenza virus by raccoons infected through contact with virus-laden water (high dose treatment).
Animal | 0 DPI | 1 DPI | 2 DPI | 3 DPI | 4 DPI | 5 DPI | 6 DPI | 7 DPI | 8 DPI | 14 DPI |
A | –a | – | 3.4* | 2.3* | 2.1* | 4.2*b | 2.7* | – | S | – |
B | – | S | – | – | – | – | 2.6* | S | S | – |
C | – | – | S | – | 3.2* | S | – | S | – | – |
D | – | – | – | – | – | – | – | – | – | – |
Nasal shedding was assessed via nasal swabs by RRT-PCR. A dash “—” indicates that no viral RNA was detected. An asterisk “*” indicates that live virus was confirmed in sample by virus isolation. Virus isolation was only attempted with samples with Ct values <38.
Because initial results were much higher than most other samples, a second RRT-PCR run was conducted. Results presented represent the mean among the two independent runs.