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| gene | forward 5′-3′ primer | reverse 5′-3′ primer |
|---|---|---|
| ACTB | GGCCCAGAGCAAGAGAGGTATCC | ACGCACGATTTCCCTCTCAGC |
| BGLAP | CTCACAGATGCCAAGCCCA | CCAAGGTAGCGCCGGAGTCT |
| Runx2 | AAATGCCTCCGCTGTTATGAA | GCTCCGGCCCACAAATCT |
| ALP | TCCTGACCAAAAACCTCAAAGG | TGCTTCATGCAGAGCCTGC |
| Col I | GCGAAGGCAACAGTCGCT | CTTGGTGGTTTTGTATTCGATGAC |
| BSP-1 | AGGAGGAGGCAGAGCACA | CTGGTATGGCACAGGTGATG |
| TGFβ-1 | AGCCCGAAGCGGACTACTAT | TCCCGAATGTCTGACGTATTG |
