Figure 2.

Kinetic discrimination of RNA sequence variants on a time scale of choice from minutes to days. Each reaction contains one HCR system: (a) H^fast, (b) H^medium, (c) H^slow. ON state: cognate RNA target X. OFF state: 1-nt sequence variant X′, 2-nt sequence variant X″, or no target. Time points: 6 min, 1 h, 10 h, and 100 h. Native PAGE poststained with SYBR Gold. Targets and scRNAs at 1 μM. Reactions run in 1× PKR at 37 °C. (d) Target sequences depicted 5′ to 3′ with nucleotide variants in orange. For each HCR system, the input domain of the detecting scRNA is depicted and the location of the output domain is indicated by an ellipsis. See Supporting Information Table S3 for scRNA sequences.