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. 2014 Aug 15;9(8):e104184. doi: 10.1371/journal.pone.0104184

Figure 1. Inhibition of MafA expression in β-cell lines or mouse islets resulted in reduced expression of Prlr and Ccnd2.

Figure 1

(A) mRNA expression of the indicated molecules in MIN6 cells transfected with MafA siRNA relative to cells transfected with control siRNA. n = 4 for each molecule, except n = 3 for MafB. Black bars: molecules detected in the transcriptome of MafA KO islets; white bars: molecules involved in β-cell function. (B) qRT-PCR analysis of prolactin signaling components in MafA KO islets relative to wild-type islets. n = 5 for Prlr; n = 3 for Jak2, Stat5A and Stat5B. (C) qRT-PCR analysis of molecules involved in the cell cycle in MafA KO islets relative to wild-type islets. n = 4 for Ccnd1; n = 5 for Ccnd2 and Ccnd3; n = 3 for Cdkn1a, Cdkn1b and Cdkn1c. (D) Immunoblots of Prlr and actin in wild-type (WT) and MafA KO (KO) islets. n = 3. (E) Immunoblots of Ccnd2 and actin in wild-type (WT) and MafA KO (KO) islets. n = 3. (F) Quantification of the results of (D) and (E). The data represent the mean ± S.E.M. in (A), (B), (C) and (F), and representative results are shown in (D) and (E). Asterisks means p<0.05.