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. 2014 Aug 15;9(8):e104407. doi: 10.1371/journal.pone.0104407

Figure 2. Infarct size.

Figure 2

MI was induced in male and female C57BL/6 mice by ligation of LAD, and immediately i.p. injected with CpG B (n = 25) or vehicle (n = 35). Hearts were reperfused after 30 minutes of ischemia. After 24 hours of reperfusion, LAD was re-ligated, area at risk was visualized by coronary perfusion of Evans-blue and viable myocardium was demonstrated by TTC-staining including all animals. Infarct size was calculated as ischemic area (IA) in percent of area at risk (AaR). Panel A: representative photograph of Evans blue and TTC-stained murine heart sections; treated with PBS or CpG B, respectively. Blue color: viable area, red color: AaR, white color: IA. Panel B: Bar-graph representation of infarct extension as calculated from IA and AaR. Each data point represents mean ± SEM.