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. 2014 Aug 15;9(8):e104407. doi: 10.1371/journal.pone.0104407

Figure 3. PCR.

Figure 3

Male and female C57BL/6 mice were subjected to I/R (black bars) or sham-operation (white bars) with i.p. injections of CpG B (sham: n = 5, I/R: n = 10) or vehicle (sham: n = 5, I/R: n = 10). After 24 hours of reperfusion, hearts were explanted and analyzed by real time RT-PCR for the expression levels of IL-1α, IL-1β, IL-1RA, CXCL1, TNFα, IFNβ, CCL2 and CD14 (Panels A–H). Each data point represents mean ± SEM. *p<0.05 vs. PBS-injection, ** p<0.01 vs. PBS-injection, ***p<0.001 vs. PBS-injection.