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. 2014 Aug 15;9(8):e104503. doi: 10.1371/journal.pone.0104503

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© 2014 Yan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A-C) Tbx18 expression in the developing ureters. A and B are whole-mount X-gal staining of Tbx18^nlacZ/+ urinary system at E14.5 and E18.5, respectively. C is a transverse section of the ureter tube at E18.5. (D) Immunostaining of α-SMA (red) on Tbx18^H2BGFP/+ ureter (transverse section) at E18.5. (E,F) Immunofluorescence analysis of Smad4 with Tbx18 lineage on Tbx18^Cre/+;Rosa26^tdTomato ureter at E16.5 and E18.5. Arrowheads indicate urothelium and arrows indicate ureter tube (A and B) or ureteral SMCs (C-F).