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. 2014 Aug 15;9(8):e104743. doi: 10.1371/journal.pone.0104743

Figure 6. Immunohistochemistry (H & E and CD31) and immunofluorescence (SIRT1, HIF-1α and VEGF) assay.

Figure 6

(A) Photographs showing SIRT1(+)-, HIF-1α (+)- and VEGF (+)-spots in joint sections of vehicle control and cilostazol (CSZ)-treated CIA mice. (B) Tibiotarsal joint tissue sections were stained with anti-CD31 antibody, and rabbit anti-human vWF (a marker of endothelial cells). Numbers of blood vessels/field and percentages of vWF stained area were analyzed in joint sections of CIA mice. Results are the means ±SEM of 4 experiments. *P<0.05, **P<0.01 vs. control; # P<0.05 vs. vehicle (Veh)-treated CIA mice. The pictures shown are representative of 4 experiments that produced similar results.