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. 2014 Aug 15;9(8):e104946. doi: 10.1371/journal.pone.0104946

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Analysis of not colonized (free of fungus) plants (negative control); (B) plants endophytically colonized by Metarhizium robertsii ARSEF 2575; and (C) not-colonized plants spiked with DTX standards (positive control). The cowpea seeds, both fungus-inoculated and control (not colonized) were incubated on moist filter paper under optimal light (16L∶8D) and temperature (25°C) conditions for 12 days at which time the germlings had developed roots, stems, cotyledons and two true leaves. DTXs were extracted from entire plants using methanol 100% and SPE-C18 cartridges.