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. 2014 Aug 15;9(8):e104946. doi: 10.1371/journal.pone.0104946

Table 2. Mean mortality (%) ± standard error of Tenebrio molitor larvae 5 days after treatment, and Galleria mellonella 3 days after treatment.

Tenebrio molitor Galleria mellonella
Fungal Isolates Conidia concentration (mL−1) Conidia concentration (mL−1)
1×107 1×105 1×107 1×105
ARSEF 3643 100.00±0.0 d 45.00±23.63 bce 100.00±0.0 f 14.58±8.33 a
ARSEF 1448 100.0±0.0 d 28.33±15.90 bcde 84.38±12.76 bdf 31.25±25.52 a
ARSEF 1885 96.67±1.7 cd 66.67±23.33 b 100.00±0.0 f 27.08±21.14 a
ARSEF 724 95.00±5.0 cd 45.00±22.55 bce 100.00±0.0 f 8.33±2.08 a
ARSEF 760 93.33±6.7 cd 13.33±7.26 ac 100.00±0.0 f 4.17±2.08 a
ARSEF 2575 93.33±6.7 cd 28.33±21.86 bc 100.00±0.0 f 20.83±20.83 a
ARSEF 1449 90.00±7.6 bcd 16.67±14.24 ac 97.92±2.08 df 8.33±5.51 a
ARSEF 782 88.33±9.3 bcd 16.67±7.26 bce 97.92±2.08 df 14.17±8.70 a
ARSEF 3641 86.67±8.8 bcde 33.33±20.28 bcde 100.00±0.0 f 53.13±38.27 a
ARSEF 2521 80.0±11.5 bcde 28.33±23.33 bce 68.75±31.25 bdefg 34.38±28.07 a
ARSEF 929 75.00±15.3 acd 16.67±14.24 ac 52.08±28.94 abd 20.83 12.67 a
ARSEF 759 66.67±20.3 abcd 3.33±3.33 ade 79.17±20.83 bcdf 16.67±9.08 a
ARSEF 552 63.33±11.7 abcd 0.00±0.0 a 89.58±10.42 bdf 0.00±0.0 a
ARSEF 2211 61.67±25.9 abd 5.00±2.89 ac 70.83±29.17 bdefg 25.00±18.75 a
ARSEF 729 46.67±14.8 abc 1.67±1.67 ade 64.58±26.60 bcdf 10.42±5.51 a
ARSEF 3918 40.00±30.6 abc 0.00±0.0 a 35.42±29.39 ab 53.13±38.27 a
ARSEF 1883 21.67±10.9 ab 0.00±0.0 a 33.33±18.52 ace 27.08±24.03 a
ARSEF 1882 16.67±14.2 ab 5.00±0.0 ac 35.42±18.52 ace 8.33±8.33 a
ARSEF 324 13.33±10.9 ae 0.00±0.0 a 31.25±15.73 acg 4.17±4.17 a
ARSEF 3479 5.00±5.0 a 1.67±1.67 ad 6.25±0.0 a 8.33±2.08 a
Control 1.67±1.7 a 1.67±1.7 ad 4.17±4.17 a 4.17±4.17 a

Bioassays were performed 3 times (using two replicates for each isolate) under controlled conditions (27°C), using new batches of larvae and conidia in each bioassay. Controls were treated with Tween 80 (0.01%) solution. Means followed by the same letter in a column do not differ statistically (P ≥ 0.05) (Kruskal-Wallis test followed by Student-Newman-Keuls).