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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Res Microbiol. 2014 May 9;165(6):429–438. doi: 10.1016/j.resmic.2014.04.005

Figure 4. Preparation, identification and characterization of PdhR.

Figure 4

(A) SDS-PAGE (12%) analysis of purified PdhR.

The molecular weights of the standard protein markers (Biorad) are labeled at the left. The N-terminal tagged PdhR protein with an expected size (~32 kDa) is indicated by an arrow.

(B) Chemical cross-linking assays for the solution structure of the PdhR protein.

Ethylene glycol bis-succinimidylsuccinate (EGS) was the cross-linker. The triangle on the top represents the addition of EGS cross linker in varied concentrations (0, 5, 10, 20, 50 μM in the right-hand five lanes [left to right]). M: Molecular weight. The monomeric PdhR protein size is predicted to be ~32 kDa, and thus the dimeric weight is ~64 kDa.

(C) MS identification of the purified PdhR protein

The tryptic peptides with hits to the PdhR sequence are given in bold underlined type.