Figure 1. Connective tissue growth factor (CCN2), epithelial membrane antigen (EMA), fibroblast activation protein (FAP), and keratin 19 (K19) expression in hepatocellular carcinomas (HCCs) of cohort 1.

A) Small nests of tumor cells are surrounded by fibrous stroma. B) Immunohistochemistry for CCN2 exhibits diffuse cytoplasmic expression in tumor epithelial cells of HCC. C–D) Immunohistochemistry for EMA reveals patchy or focal expression pattern in tumor epithelial cells of HCC. E–F) K19 is focally positive in the periphery of tumor nests. (G–H) FAP is expressed in the cytoplasm of cancer-associated fibroblasts of tumor stroma. (Scale bars represent 100 µm.)