Table 1. Biochemical properties of scaffold-free, engineered tissues.

	Control	TGF-β1 [0.1 nM]	IGF-1 [10 nM]	Insulin [100 nM]
	(n = 7)	(n = 5)	(n = 8)	(n = 8)
Total DNA (µg/construct)	30±4a	4.4±0.4a	58±6	55±6
Total PG (µg/construct)	1918±374a	95±42a	6258±880	6356±1071
Total Collagen (µg/construct)	2345±903a	504±137a	5777±1780	4938±1562
Wet Weight (mg)	87±13a	23±5a	197±20	183±14
Dry Weight (mg)	9±1a	1.9±0.4a	17±1	18.2±0.8
Water Content (%)	89.6±0.5	91.9±0.4	91.1±0.2	90.6±0.2

Biochemical Properties of Engineered Elastic Cartilaginous Tissues. Data presented as mean ±SEM.

PG: Proteoglycans.

^aSignificantly different from all other groups (p<0.01).