Figure 3. Production of catalase and integrity of glycerol metabolism operon in M. gallisepticum transformants.
(a) Immunoblot of M. gallisepticum transformants 56A and 56C and M. gallisepticum Rlow with anti-6X-His antibodies. (b) PCR products using primers for the putative glpFOK operon of M. gallisepticum. L, ladder with bands at 10, 8, 6, 5, 4, 3, 2.5, 2, 1.5, 1, 0.7, and 0.5 kb; A, M. gallisepticum Rlow; B–D, M. gallisepticum transformants 55A–C; E–F, M. gallisepticum transformants 56A and C; G, M. iowae serovar K.