Figure 2. Effects of DEX on GSK-3β activity during myogenic differentiation.
(A) C2C12 myoblasts were induced to differentiate in the absence (CON) or presence of 10−5 M DEX. Phosphorylation of GSK-3β at serine 9 at the indicated time points was determined using Western blot analysis. (B) Differentiating C2C12 myoblasts were incubated with DEX and RU-486 (10 µM), alone or in combination, or control DM (CON). Phosphorylation of GSK-3β at serine 9 at the indicated time points was determined using Western blot analysis. The data are shown as the means ± SEM of three independent experiments. *P<0.05 compared to control group (without DEX or RU-486 in DM).