Figure 1. PrP mRNA in sCJD brain samples. RNA average expression levels of PrP in the frontal cortex (A) and cerebellum (B) of control, sCJD MM1 and sCJD VV2 subtypes, as determined by TaqMan PCR assays. Values for PrP mRNA are normalized using XPNPEP1 (A and B, upper panels) and GUSβ (A and B lower panels) as internal controls (housekeeping genes). Reduced PrP mRNA is seen in the frontal cortex in sCJD MM1 and cerebellum in sCJD VV2. Similar results are obtained with the housekeeping genes. *P > 0.05; **P > 0.01, control vs sCJD. AU: arbitrary units.