Table 1. Summary of the findings related to PrP levels, PrP^sc deposition, spongiform degeneration, neuron loss, astrogliosis and microgliosis in sCJD cases in the present series.

	sCJD MM1		sCJD VV2
	Frontal cortex	Cerebellum	Frontal cortex	Cerebellum
PrP levels
PrP mRNA	Decreased	Unchanged	Unchanged	Decreased
PrPc + PrPsc	Decreased	Unchanged	Unchanged	Increased
PrPsc pattern	Synaptic	Synaptic	Synaptic + Perineuronal + Plaque-like	Synaptic + Plaque-like
PrPsc	1	1	2	2
Neuropathological hallmarks
Spongiform degeneration	1–2 small vacuoles	1–2 small vacuoles	2 small vacuoles	2–3 medium vacuoles
Purkinje loss	-	-1	-	1–2
Granule cell loss	-	0–1	-	2
Neuronal loss (frontal cortex)	1–2	-	1–2	-
Astrogliosis	2–3	1–2	2	2
Microgliosis	2–3	2	2	2–3

Parameters were scored as 0 = absent, 1 = mild, 2 = moderate, and 3 = severe. Mild was considered when at least a 200-fold magnification was needed to observe the alterations, moderate when a 100× magnification was needed, and severe when they were already seen at 20x .