Figure 4.hsp104-G254D and hsp104-G730D display different levels of non-prion disaggregation. The thermotolerance of HSP104, hsp104Δ, hsp104-G254D, and hsp104-G730D cells was tested. Cells were first grown at 37 °C in liquid culture to induce HSP104 expression, heat-shocked at 50 °C for 10 to 30 min as indicated, and then spotted on rich media. Control cells (No Heat) were plated without heat shock. Thermotolerance assays were repeated four times and all showed similar results.