Figure 6.hsp104-G730D can propagate multi-dot high [RNQ+]. hsp104-G730D cells were mated to s.d. low (low), medium (med), high (high), very high (v.high) and m.d. high (m.d. high) [RNQ+] and then sporulated. The hsp104-G730D haploid progeny from four separate tetrads of each variant mating, along with the unmated [RNQ+] variants as controls (WT), were analyzed by SDD-AGE and western blot.