Table 1. Hsp104 mutants display defects in ATP hydrolysis under physiological salt conditions.

	Physiological*	Low†
WT	0.225 ± 0.0397	0.732 ± 0.0543
G254D	0.0325 ± 0.00177	0.211 ± 0.00177
G730D	0.00583 ± 0.00439	0.677 ± 0.0321

Numbers represent the average initial rate (nmol·µg⁻¹·min⁻¹) of ATP hydrolysis for WT Hsp104, Hsp104-G254D, and Hsp104-G730D. ATPase assays were measured by the Malachite Green assay. Rates were calculated from three separate experiments from 2 different preparations of purified protein. *Buffer contains 150 mM NaCl. ^†Buffer contains only 15 mM NaCl.