Table 1. Particle radii of wt hPrP 121–231 and its mutants determined by dynamic light scattering (DLS).
| RH (nm) | |||||
|---|---|---|---|---|---|
| Protein | RHcalc (nm) | 0 min | 1 min | 30 min | 60 min |
| wt hPrP121–231 | 1.8 | 1.9 ± 0.1 | >100 | >100 | n.d.a |
| hPrP121–231 M129T | 1.8 | 1.9 ± 0.1 | >100 | >100 | n.d.a |
| v-hPrP121–231 | 1.8 | 1.7 ± 0.4 | 17.3 ± 2.7 | 15.0 ± 2.5 | n.d.a |
Metal-induced oxidation of hPrP121–231 and its mutants was performed at 37 °C. After indicated time periods the hydrodynamic radii of the proteins were analyzed. RH, hydrodynamic radius; RHcalc, theoretical hydrodynamic radius calculated from the molecular mass; n.d.a, no significant count rate detected.