Fig. 6.

GAP40 is lost from the residual body and appears in the ELC during daughter parasite emergence and maturation. (A) Colocalization of the IMC protein GAP40 (green) and plasma membrane protein SAG1 (red) at various stages throughout the T. gondii replicative cycle. Selected images from fixed specimens are shown, approximately corresponding to times t = 0 (i), 30 min (ii), 150 min (iii), 190 min (iv), 200 min (v), 225 min (vi) and 360 min (vii). (B) Cartoon illustrating the relative location of IMC (green) and the parasite plasma membrane (red); arrows in emerging parasites suggest recycling of IMC from the maternal residual body to the daughter IMC. (C) GAP40 (green) traffics to the IMC through the ELC during both elongation (de novo synthesis; ii) and maturation (salvage of the maternal IMC; vi). Arrows indicate the appearance of GAP40 in the Golgi and ELC region of daughter parasites shortly after emergence from the mother (vi); arrowheads indicate the residual body. Scale bars: 5 µm.