Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Amino Acids. 2014 Jun 15;46(9):2231–2239. doi: 10.1007/s00726-014-1777-0

Fig. 2.

Fig. 2

(A) IEC-6 cells (500,000/well) were plated in control, DFMO, or DFMO + 5 μM Spm containing medium and incubated at 37°C. Total cell counts were determined using a coulter counter. (B) Growth rates were determined by calculating mean doubling times. (C) Cells grown as described above for 4 days were used to determine antizyme-1 and actin by western blot analysis. Values are means ± SE of triplicates. *, p < 0.05 compared with Control. #, p < 0.05 compared with Control and DFMO. Blots shown are representative of 3 observations.