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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Amino Acids. 2014 Jun 15;46(9):2231–2239. doi: 10.1007/s00726-014-1777-0

Fig. 4.

Fig. 4

(A) IEC-6 cells (500,000/well) were plated in DFMO, or DFMO + Put (10 μM), DFMO + Put + Spd (5 μM) or DFMO + Put + Spm (5 μM) containing medium and incubated at 37°C. Cells were photographed on day 2 and day 4 using a phase contrast microscope. 20X images are shown for each group. Representative images from three experiments carried out in triplicate are shown. (B) Cells grown as described above for 4 days were used to determine antizyme-1 and actin by western blot analysis. Blots shown are representative of 3 observations.