Figure 4.

Gyrase activity assay after treatment of seedcake extract. Part A. Analysis of W92 seedcake extracts activity. Lane 1- negative control (without gyrase and extract); lane2- positive control (gyrase, without extract); lane 3- total inhibition control (novobiocin 25 μg/ml, gyrase, without extract); lane 4- reaction mixture with 10 mg/ml dried seedcake extract; lane 5- reaction mixture with 30 mg/ml dried seedcake extract; lane 6- reaction mixture with 50 mg/ml dried seedcake extract; lane 7- reaction mixture with 75 mg/ml dried seedcake extract. Part B. Analysis of control plant seedcake extracts activity. lane 8- positive control (gyrase, without extract), lane 9- reaction mixture with 10 mg/ml dried seedcake extract; lane 10- reaction mixture with 30 mg/ml dried seedcake extract; lane 11- reaction mixture with 50 mg/ml dried seedcake extract.