Table 3.
|
WT seedcakes dried extract |
W92 seedcakes dried extract |
||
---|---|---|---|---|
50 mgDW/ml preparation | 50 mgDW/ml preparation | 30 mgDW/ml preparation | 10 mg/ml preparation | |
Total phenolics content [mg/ml] |
24.26 ± 0.03 |
31.59 ± 0.05 |
18.45 ± 0.03 |
6.03 ± 0.06 |
IC-50 [μl]* |
27.5 ± 0.04 |
10.56 ± 0.03 |
18.15 ± 0.03 |
26.36 ± 0.04 |
K. pneumoniae ATCC 700603** |
64% ± 3.03 |
100% ± 0.00 |
98% ± 0.01 |
72% ± 0.93 |
S. aureus ATCC 29213** |
52% ± 2.01 |
100% ± 0.01 |
93% ± 1.03 |
60% ± 0.63 |
P. aeruginosa ATCC 27853** |
46% ± 3.50 |
100% ± 0.00 |
100% ± 0.02 |
85% ± 1.05 |
E. coli ATCC 25922** | 75% ± 1.89 | 100% ± 0.23 | 87% ± 1.83 | 48% ± 1.82 |
The antimicrobial activity of seedcake preparations were tested on reference bacterial strains. The low concentration of seedcake preparation from control plants (10 mg/ml and 30 mg/ml) do not show any antibacterial properties (data not shown).
*The antioxidant potential (IC50) was defined as the amount (μl) of preparation that inhibits luminol chemiluminescence by 50%. **The presented results are the inhibition rate compared to the untreated bacteria (% of growth inhibition) after 6 h treatment. The results are the mean values ± SD (n = 5).