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. 2014 Jun 12;111(4):716–725. doi: 10.1038/bjc.2014.325

Figure 5.

Figure 5

Verapamil and MDM2–p53 antagonists Nutlin-3, NDD0005 and MI-63 sensitise high MDR-1-expressing neuroblastoma cells but not low MDR-1-expressing cells to vincristine-mediated apoptosis. Western blot analysis showing the levels of cleaved caspase-3 as a marker of apoptosis in (A) SKNBe2C, (B) SHSY5Y and (C) SKNAS cells treated for 72 h with 1 × their respective 72-h vincristine GI50 concentrations (SKNBe2C 32.8 nM; SHSY5Y 8.2 nM; SKNAS 1.5 nM) or 2 μM verapamil, Nutlin-3, NDD0005, MI-63 or RG7388 alone, or in combination. GAPDH was used as a loading control.