Figure 4.

Mammary tumour cell lines originating from PyVmT;Abcc10 null cells are sensitised to cytotoxic ABCC10 substrates. (A) Images of colonies from Abcc10^−/− vs Abcc10^+/+ cells following paclitaxel or docetaxel treatment (the analysis with controls is presented on graphs under images). (B) Microtubule staining of Abcc10 wild-type and Abcc10 null cell lines after incubation with 3, 10, or 25 nM of docetaxel; tubulin (red), nuclei (blue). Scale bar, 100 μm. (C) Images from wound-healing assays assessing the mobility of wild-type and Abcc10^−/− lines after docetaxel treatment (the analysis with controls is presented on graphs under images). (D) Accumulation of 0.1 μM [³H]-docetaxel in Abcc10^+/+ cells (WT, black triangle) compared with the mammary tumour cell lines derived from Abcc10-disrupted mice (KO, white triangle) was observed. Error bars denote mean±s.e.m. Statistical significance was determined by two-sided Student's t-test.