Table 1. Cell cycle distribution in H1299 bystander cells cultured in 18-h conditioned medium.
| Untreated control | 18-h SCM | 18-h RCM | |
|---|---|---|---|
|
24 h | |||
| G1% | 53.46±4.73 | 51.60±1.04 | 51.45±2.75 |
| S% | 34.17±3.47 | 39.92±0.76 | 34.21±2.70 |
| G2/M% |
12.36±1.34 |
8.48±0.49 |
13.33±0.62* |
|
48 h | |||
| G1% | 52.49±2.10 | 51.85±3.06 | 50.58±3.05 |
| S% | 34.20±2.36 | 38.36±2.24 | 36.95±2.14 |
| G2/M% |
13.31±0.89 |
9.79±0.96 |
12.47±1.01 |
|
72 h | |||
| G1% | 55.49±3.34 | 49.76±3.95 | 52.20±4.16 |
| S% | 33.06±2.39 | 40.64±3.34 | 37.28±3.33 |
| G2/M% | 11.46±2.05 | 9.60±0.86 | 10.52±1.63 |
Abbreviations: RCM=radiation-conditioned medium; SCM=sham-radiation-conditioned medium.
The cells were cultured in 18-h conditioned medium for 24, 48 and 72 h, then the cell cycle distribution was determined by flow cytometry. * represents P-value <0.05 vs SCM.