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. 2014 Jul 10;111(4):807–816. doi: 10.1038/bjc.2014.372

Figure 3.

Figure 3

The effects of reduced expression of HOXD10 in high-HOXD10-expressing OPL and HNSCC cells. (A). Expression of HOXD10 assessed using qPCR and WB, confirming reduction in expression of HOXD10 in the transfected cells, compared with scrambled siRNA-transfected controls. β-actin is used as a loading control in WB. (B–D) Reducing HOXD10 expression in D35 and T5 cells reduces migration in the Transwell assay (B), reduces adhesion to fibronectin (C) and reduces proliferation as assessed by the MTS assay over 96 h (D). (E) Reducing HOXD10 expression in D35 and T5 increases invasion into matrigel. *P<0.05, **P<0.01, ***P<0.001. Each assay was conducted in triplicate and repeated three times. The data show the mean of triplicate repeats±s.e.m.