Figure 6. DDX17 directly binds miRNA stem loops in human U2OS cells.
A. Normalized CLIP-seq signal (TPKM, tags per kilobase of pre-miRNA per million CLIP-seq reads) in pre-miRNA hairpin loci with CLIP signal extracted from miRBase. Linear regression of infected TPKM on uninfected TPKM is plotted, R2=0.79. B. Scatterplot of miRNAs that are bound; normalized pre-miRNA expression (RPKM) from small RNA-seq and the mean of normalized CLIP-seq signal (TPKM) between infected and uninfected U2OS cells are plotted, R2=0.001. C. Alignment clusters overlapping miRBase pre-miRNA hairpin loci on the UCSC genome browser with uninfected cells colored black, and infected cells colored red. D. RNA map of DDX17 CLIP signal in pre-miRNA hairpins. Fraction of 160 hairpins bound is plotted at single-nucleotide resolution relative to the center of the stem-loop. See also Tables S3 and S4.