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Study design. After four days of differentiation, cells were treated for two days (three times per day) with differentiation media (DM) and vehicle (10 mM Tris-NaCl), 10 ng/ml recombinant GDF-8 MSTN, 10 ng/ml MSTN and 10 μM leucine, 10 ng/ml MSTN and 10 μM of HMB, or 10 ng/ml MSTN and 10 μM creatine monohydrate.
