Figure 1. Generation of hGSTP1::LacZ mice.
The construct used to generate the of hGSTP1::LacZROSA26 mouse. Approximately 2.6 kb of the human GSTP1 promotor and the entire GSTP1 gene (~2.5kb) were cloned, fused in-frame with lacZ, into the ROSA26 gene locus by homologous recombination in C57BL/6 embryonic stem cells. Positions of known transcription factor binding sites, and previously reported regulators of gene induction or repression, are shown: cAMP/CRE; NF-kB; RA(32); AP-1/Sp1(18, 48, 49); Insulin(32, 50); p53(31); RA/RARE(50).