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. Author manuscript; available in PMC: 2014 Aug 16.
Published in final edited form as: J Am Chem Soc. 2013 Feb 14;135(8):2999–3010. doi: 10.1021/ja306361q

Figure 5. Temperature dependence of Kd in WT and E-to-Q SecA.

Figure 5

The Mg-MANT-ADP-binding affinity of WT (●) or mutant (■) variants of BsSecA (panel A) or EcSecA (panel B) were measured at temperatures from 5–30 °C in KEMT buffer (pH 7.6) using the fluorescence anisotropy assay shown in Fig. 2 with 900 nM Mg-MANT-ADP.