Skip to main content
. Author manuscript; available in PMC: 2014 Aug 16.
Published in final edited form as: J Am Chem Soc. 2013 Feb 14;135(8):2999–3010. doi: 10.1021/ja306361q

Figure 6. Isothermal titration calorimetry (ITC) reveals that large entropy losses accompany nucleotide binding to E208Q-BsSecA and WT-EcSecA.

Figure 6

ITC experiments were performed at 25 °C in TKM buffer (pH 8.0). Mg-ADP was titrated onto 20 μM WT-BsSecA (panel A), 30 μM E208Q-BsSecA (panel B), or 10 μM WT-EcSecA (panel C). Table 1 gives the thermodynamic parameters inferred from curve-fitting these data.