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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Andrology. 2014 Jul 23;2(5):695–701. doi: 10.1111/j.2047-2927.2014.00243.x

Figure 2. GPER-expression in isolated human testicular peritubular cells.

Figure 2

Result of a representative RT-PCR experiment (A) using HTPCs of three different donors (1–3). A single band at the expected size of 120 bp is depicted, which upon sequencing was found to correspond to human GPER. Negative control (−) including RNA instead of cDNA showed no band.

Western blot (B) of GPER was performed with 10 μg of total proteins extracted from 3 different HTPCs (1–3) showing a band of 42 kDa. The membrane was stripped and reprobed for GAPDH (37 kDA) as a loading control.

Analysis of cell number and their viability using CASY (C) revealed a significant lower cell count in G1 stimulated HTPCs after 24 h compared to vehicle treated cells. n = 5; Student’s t-test, p < 0.05.