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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Clin Cancer Res. 2014 Jun 11;20(16):4262–4273. doi: 10.1158/1078-0432.CCR-13-2627

Fig 5. CAR TIL cytolytic and cytokine secreting functions recover after rest away from tumor. DGK and pSHP1 expression is increased in hypofunctional CAR TILs.

Fig 5

Human TILs isolated from flank EMMESO tumors at Day 39 and “rested” away from tumor for 24 hours demonstrated significant recovery in their (A) EMMESOffluc tumor killing ability and (B) their ability to secrete IFNγ in response to EMMESOffluc tumor. (C) Compared to freshly harvested EMMESO TILs, resting away from tumor led to recovery of antigen-specific signaling function of EMMESO TILs as measured by detection of phosphorylated ERK. (D) DGKa and DGKz and pSHP1 significantly elevated in TILs freshly isolated from EMMESO. Expression of both isoforms of DGK as well as pSHP1 decreased dramatically after over night rest of TILs.