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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Inflamm Bowel Dis. 2014 Sep;20(9):1530–1547. doi: 10.1097/MIB.0000000000000144

Fig. 3.

Fig. 3

Comet assays. A) Normal human colon cells (NCM 460) were fed with fresh complete media alone, as a control or B) with 150 μM Hb for 4 h followed by a comet assay to assess DNA damage (DNAD) using a denaturing electrophoresis. C (a) (b) and (c), show an interpretation of the intensity of DNAD: a = undamaged cells, b = damaged cells and, c = severely damaged cells. D) Quantification of DNAD.