Table 2.

Depicts data results of NCM 460 cells treated with Hb(n) and Hb, individually, are mediators for ROS-dependent cell formation. Effects of Hb(n) and Hb, individually, on ROS with concentration-time dependent manner of 10 μM and 100 μM at 2 h, 6 h, 12 h and 24 h analytical points. The generation of ROS was measured and quantified by the production of 8-OHdG. Data are given as mean ± S.D and S.E. Statistical significance was determined by Turkey test after one-way ANOVA. Unless stated otherwise calculated differences were regarded as significant for a probability value of less than 0.05.

Hemoglobin alpha (Hb-α)
Hb-α 10				Hb-α 100			p-value
Time	Mean	Standard Deviation	Standard Error	Mean	Standard Deviation	Standard Error	Hb-α, 10 μM vs. 100 μM	TbHP vs. Hb-α 10 μM	TbHP vs. Hb-α 100 μM
0 h	2.25	0.43	0.22	1.75	0.43	0.22	0.1817	1.0000	0.0032
2 h	5.25	0.43	0.22	5.00	0.71	0.35	0.3910	0.005	0.0042
6 h	12.50	1.12	0.56	8.00	0.71	0.35	0.0006	0.04	0.0022
12 h	25.50	2.06	1.03	15.75	1.92	0.96	0.001	0.0003	1.0000
24 h	46.75	5.36	2.68	31.25	7.76	3.88	0.0114	0.0005	0.0234

Hemoglobin Beta (Hb-β)
Hb-β 10 μM				Hb-β 100 μM			p-value
Time	Mean	Standard Deviation	Standard Error	Mean	Standard Deviation	Standard Error	Hb-β, 10 μM vs. 100 μM	TbHP vs. Hb-β 10 μM	TbHP vs. Hb-β 100 μM
0 h	2.50	0.50	0.25	1.75	0.83	0.41	0.06	0.006	0.003
2 h	6.25	0.43	0.22	4.50	0.50	0.25	0.006	0.008	0.005
6 h	15.00	2.45	1.22	7.50	1.50	0.75	0.0014	0.2152	0.0001
12 h	33.50	4.97	2.49	14.25	1.30	0.65	0.0029	0.002	0.0577
24 h	62.00	9.41	4.70	22.00	2.55	1.27	0.0022	0.001	0.0663

Hemin
Hemin 10 μM				Hemin 100 μM			p-value
Time	Mean	Standard Deviation	Standard Error	Mean	Standard Deviation	Standard Error	Hm, 10 μM vs. 100 μM	TbHP vs. Hm 10 μM	TbHP vs. Hm 100 μM
0 h	2.75	0.83	0.41	2.50	0.50	0.25	0.3910	0.01	0.006
2 h	4.25	0.43	0.22	3.75	0.83	0.41	0.1817	0.004	0.002
6 h	5.00	0.71	0.35	5.25	0.83	0.41	0.3910	0.0007	0.001
12 h	8.75	1.48	0.74	11.25	1.79	0.89	0.02	0.0004	0.0061
24 h	7.50	1.66	0.83	13.25	1.30	0.65	0.0002	0.001	0.01

Hemoglobin
Hb 10 μM				Hb 100 μM			p-value
Time	Mean	Standard Deviation	Standard Error	Mean	Standard Deviation	Standard Error	Hb, 10 μM vs. 100 μM	TbHP vs. Hb 10 μM	TbHP vs. Hb 100 μM
0 h	2.25	0.43	0.22	2.50	0.50	0.25	0.3910	1.0000	0.0060
2 h	9.25	2.17	1.08	7.50	0.50	0.25	0.2126	0.0006	0.0154
6 h	17.75	2.49	1.24	6.75	0.83	0.41	0.002	0.006	0.0006
12 h	32.50	3.04	1.52	14.75	0.43	0.22	0.002	0.0003	0.4228
24 h	57.00	4.90	2.45	25.50	2.06	1.03	0.0009	1.0000	0.0318