Figure 2.

(a) Chemical structures of SV119 and SW120 that bind specifically to the sigma-2 receptor. (b) The relative geometric mean fluorescence intensities (GMFI) recorded from the CSCs and non-CSCs. (c) Flow cytometry analyses of the CSCs that were pre-treated with SV119 for 1 h and then incubated with SW120 (50 nM) for 0.5 h. Cells incubated with PBS were used as a control. (d) Flow cytometry analyses of the non-CSCs that were pre-treated and incubated using the same protocol as in (C). The CSCs (ALDHhi population) and non-CSCs (ALDHlow population) derived from the mammospheres were labeled using the ALDEFLUOR kit and separately collected using the BD FACSAria III cell sorter.