Figure 1. IDH1 mutation is associated with a drop in BCAT1 activity and expression in our cell model.

(A) BCAT1 enzymatic activity, as measured by a spectrophotometric assay, is significantly decreased in mutant IDH1 cells as compared to wild-type cells (*p<0.002; n=4 per cell line). (B) Western blots of the BCAT1 enzyme, showing the drop in BCAT1 protein levels in U87IDHmut cells when compared to U87IDHwt.