Table 1. Enzymatic activities, protein levels and mRNA levels for the four enzymes catalyzing the α-KG to glutamate conversion.

Enzymatic activities, protein levels and mRNA levels were measured by spectrophotometric assays, western blot and qRT-PCR analysis, respectively (first row: enzyme; second row: corresponding gene).

Enzyme	BCAT1	AST1	AST2	GDH1	GDH2	ALT1	ALT2
Gene	BCAT1	GOT1	GOT2	GLUD1	GLUD2	GPT	GPT2
Activity levels (% of U87IDHwt)	68 ± 7**	83 ± 10*		69 ± 15*		b.d.
Protein levels (% of U87IDHwt)	38 ± 12**	55 ± 11*	112 ± 30	b.d.	131 ± 33	103 ± 18	131 ± 32
mRNA levels (% of U87IDHwt)	48 ± 19**	73 ± 9**	79 ± 5**	91 ± 18	b.d.	b.d.	132 ± 30

All values are for U87IDHmut cells and expressed in percent of U87IDHwt levels (b.d.= below detection level; *p<0.05;**p<0.01; n=3 per protein per cell line; n=9 per gene per cell line).