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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Dev Biol. 2014 Jul 1;393(1):44–56. doi: 10.1016/j.ydbio.2014.06.021

Figure 1. Geminin expression and excision by Mox2-Cre during embryogenesis.

Figure 1

(A) Geminin is predominantly expressed in the neural tube at E10.5 (in situ hybridization (ISH), left panel) and E14.5 (immunohistochemistry (IHC), center). IHC for Geminin on an E11.5 transverse section through the spinal neural tube demonstrates strong staining in the ventricular region of the neural tube (right). DAPI counterstain in blue. (B) Schematic of endogenous and conditional Geminin alleles. Exons are black boxes, introns are lines, red arrowhead indicates loxP sites, and black arrow indicates transcription start site. (C) Mox2-Cre is expressed throughout the E9.5 embryo. Transverse section shows broad β-galactosidase staining. (D) Geminin IHC shows efficient excision by Mox2-Cre in the spinal neural tube of mutant (Mox2Cre;Gmnnflox/flox) embryos by E10.5. Scale bars=100 μm.