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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Hum Mutat. 2014 Jul 17;35(9):1101–1113. doi: 10.1002/humu.22602

Figure 4. TorsinA role in the secretion of sfGFP.

Figure 4

The expression of torsinA variants and torsinA knock-down did not affect the secreted levels of sfGFP (n=3). (A and B) TorsinAwt in 293T cells was down-regulated by siRNA for three days followed by transfection with sfGFP for 10 hr. siRNA reduced torsinAwt levels by >50%, yet levels of sec-sfGFP remained unaltered relative to levels of retained sfGFP. (C and D) 293T cells expressing torsinAwt or torsinA variants (n=3) also did not display an effect on sec-sfGFP levels relative to retained sfGFP levels, suggesting that torsinA does not function in the regulation of ER exit sites in the secretory pathway.