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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Dev Biol. 2014 Jun 20;393(1):149–159. doi: 10.1016/j.ydbio.2014.06.011

Figure 4. scl functions downstream of etv2 during neutropoiesis in the ALPM.

Figure 4

(A-D) scl RNA can rescue anterior mpx expression in etv2 knockdown embryos, as analyzed at 24 hpf. mpx expression in etv2 morphants (B, n=19/19) is decreased as compared to the wild type siblings (A, n=26/26). Injection of scl RNA rescues mpx expression in etv2 morphants (C, n=23/29). All embryos are positioned with anterior to the left and dorsal side up. (E) Morpholino knockdown of scl reduces the induction of mpx by etv2 DNA, as analyzed by real-time RT-PCR at the 12-somite stage. mpx expression is increased in etv2 DNA injected embryos, relative to uninjected controls but is not changed significantly when etv2 DNA is co-injected with scl MO. Relative expression levels have been normalized to EF1α expression. Asterisk denotes statistical significance vs. etv2 DNA over-expression (p<0.01) as analyzed by one-way ANOVA test with pair-wise comparison. Error bars correspond to ± standard deviation.. (n=3).