Fig. 4.

Cav-1 interacts with Fas after hyperoxia, partially via ROS and palmitoylation. (A) Blocking ROS with NAC (30 nM) partially eliminated Cav-1–Fas interaction. Beas-2B cells were pretreated with an NAC or PBS control, and then after 30 min, the cells were exposed to hyperoxia (4 h). Cell lysates were then collected, and co-IP assays were performed to determine Cav-1–Fas interactions. (B) Cav-1–Fas interaction after hyperoxia is mediated by palmitoylation. Beas-2B cells were pretreated with 2-Br (1 µM), and then after 30 min, the cells were exposed to hyperoxia (4 h). The Cav-1–Fas interaction was determined by using co-IP assays, as described previously. All blots represent three repeats. *P<0.05.