Skip to main content
. Author manuscript; available in PMC: 2014 Aug 17.
Published in final edited form as: Methods Mol Biol. 2014;1123:191–221. doi: 10.1007/978-1-62703-968-0_14

Fig. 6.

Fig. 6

Alignment of native endonuclease sequences. Alignment of a well-characterized reference sequence like I-OnuI is used to define the N- and C-terminal domains of the I-PanMI enzyme. The location of the second LAGLIDADG helix is used to guide the placement of the -SGT-tripeptide linker. Counting backwards from the residue at the top of the second LAGLIDADG helix (marked residue “0” in the magnified inset ), the -SGT-linker is substituted at positions 3 through 5 (marked with a bracket). This location was chosen as a region where there are minimal interactions between the native linker and the adjacent protein domain