Table 2.

Validation for the quantitative determination of twelve standard compounds using RP-HPLC.

Peak no.	Compound	Test range (μg/mL)	Regression equation	Precision experiment		Repeatability		Recovery experiment
				Area of peak (mAU∗min)	RSD (%)	Area of peak (mAU∗min)	RSD (%)	Average recovery rate (%)	RSD (%)
1	Chlorogenic acid	1~100	y = 7.747x − 1.0908  (R 2 = 0.9998)	377.97 ± 8.62	1.50	150.20 ± 1.65	2.38	99.87 ± 0.35	0.35
2	Epicatechin	10~500	y = 1.878x − 1.3776  (R 2 = 0.9999)	160.80 ± 2.41	1.11	265.33 ± 3.32	1.65	99.38 ± 1.65	1.66
3	Rutin	10~500	y = 22.356x + 55.311  (R 2 = 0.9999)	1042.93 ± 7.69	1.07	1944.37 ± 21.67	0.98	102.13 ± 0.88	0.86
4	Hyperoside	50~1000	y = 41.138x − 436.10  (R 2 = 0.9998)	865.77 ± 9.38	0.76	3573.98 ± 36.75	1.03	98.84 ± 0.93	0.94
5	Trifolin	1~50	y = 49.53x − 0.2025  (R 2 = 0.9999)	1000.50 ± 7.06	0.71	1125.80 ± 7.01	0.81	100.81 ± 1.02	1.01
6	Quercitrin	10~500	y = 63.459x − 171.41  (R 2 = 0.9994)	3641.90 ± 27.59	1.08	5210.20 ± 11.06	0.16	99.90 ± 0.31	0.31
7	Kaempferol-3-rhamnoside	1~100	y = 41.826x + 1.308  (R 2 = 0.9999)	2350.82 ± 35.73	0.74	753.97 ± 10.68	1.42	100.60 ± 0.91	0.91
8	Quercetin	1~50	y = 14.392x + 15.374  (R 2 = 0.9997)	182.53 ± 6.35	1.06	100.10 ± 4.83	0.92	101.35 ± 0.89	0.88
9	Nitidine chloride	0.1~5	y = 166.49x + 33.656  (R 2 = 0.9993)	140.71 ± 6.47	1.17	130.11 ± 5.52	1.11	99.75 ± 1.02	1.02
10	Chelerythrine	0.1~5	y = 175.41x + 0.7376  (R 2 = 0.9993)	114.54 ± 7.31	1.51	85.16 ± 6.22	1.56	103.76 ± 1.51	1.45
11	Xanthyletin	0.1~5	y = 98.498x + 67.619  (R 2 = 0.9995)	110.68 ± 8.35	1.12	93.52 ± 13.07	1.03	98.37 ± 1.04	1.06
15	Sesamin	10~500	y = 4.1445x + 107.11  (R 2 = 0.9991)	223.05 ± 8.03	1.32	215.04 ± 0.81	2.94	101.52 ± 1.32	1.75

Values were expressed as mean ± SD (n = 6).