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Myocardial expression of cytokine mRNA. Real-time qPCR analysis of mRNA expression in CCC and NIC myocardium. After normalization to GAPDH mRNA, relative increase was plotted in comparison to N group and data were calculated with the 2−ΔΔCt method, as described in Methods section. The horizontal bar stands for the median. Dotted lines indicate twofold increase or decrease of expression as compared with the control group.
